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. 2015 Jul 21;7(7):2685–2700. doi: 10.3390/toxins7072685

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Sodium dodecyl sulfate polyacrylamide gel electrophoresis of the purified rice UDP-glucosyltransferase OsUGT79 and sucrose synthase AtSUS1. Lane 1: Precision Plus Protein Standard (Bio-Rad); lane 2: immobilized metal ion affinity chromatography (IMAC)-purified OsUGT79 (UGT-cHis₆, 52 kDa); lane 3: IMAC-purified OsUGT79 (nHis₆-MalE-UGT, 95 kDa); lane 4: nHis₆-MalE-UGT after size exclusion chromatography; lane 5: IMAC-purified AtSUS1 (93 kDa).