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. 2015 Jul 28;5:12440. doi: 10.1038/srep12440

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Copyright © 2015, Macmillan Publishers Limited

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(A) The effective dose of ACEA against OGD/R evaluated by WST-8 (n = 8). Cell injury was evaluated in terms of WST-8 cell viability (B), (n = 8), LDH release (C), (n = 6), apoptosis rate (D, E), (n = 5) and intracellular ROS level (F, G), (n = 6) at 24 h after reoxygenation. Scale bars = 20 μm. Data represent mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; n.s.: no significance. OGD/R: oxygen-glucose deprivation/reoxygenation; WST-8: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt; Hemo: hemopressin; LDH: lactate dehydrogenase; ROS: reactive oxygen species.