Figure 2.

A. veronii possess gene for utilizing iron from heme. The heme receptor locus from HM21 is shown (A). The solid line indicates length of the gene. The three regions used for complementation with a Tn7 are represented by the dashed lines, the upper dashed line (SHC1), the middle dashed line (SHC2), and the bottom dashed line (SHC4). (B) The growth yield of Hm21R (blue); H-890 (red), the hgpB mutant; S-497 (yellow), the siderophore mutant; and SH-894 (green), the double mutant was determined by measuring the OD₆₀₀ 24 h after inoculation in LIM, LIM (150 μM EDDA), or LIM (150 μM EDDA and 50 μg/ml hemin). The statistically significant difference between the parent strain and each mutant was performed using a Mann–Whitney test. This revealed the requirement of the hgpB for utilizing hemin as an iron source in the presence of EDDA.