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. 2015 Jul 28;6:763. doi: 10.3389/fmicb.2015.00763

Figure 3.

Figure 3

In vitro and in vivo proliferation of iron utilization mutants. A time course of growth in blood of Hm21R (blue); H-890 (red), the hgpB mutant; S-497 (yellow), the siderophore mutant; and SH-894 (green), the double mutant at 18, 24, 42, and 72 h is shown (A). This revealed that in blood either viuB or hgpB suffice for growth. The symbols represent the average of 3 leech gut samples (A). Colonization of iron utilization mutants and complemented strains within the leech crop is shown (B). Each symbol represents the colonization level in one leech. The statistically significant difference between the parent strain and each mutant was performed using a Mann–Whitney test. Bonferroni corrections were preformed and the critical threshold is 0.007. N/S, not significant.