Table 1.
Bacterial strains and plasmids.
| Bacterial strains and plasmids | Characteristics | Source or reference |
|---|---|---|
| A. VERONII STRAINS | ||
| HM21R | Parent strain, Rf r | Graf, 1999a |
| HM21RS | Parent strain, Rf r, Smr | Graf, 1999a |
| S-497 | HM21R, viuB::mTn5, Rfr, Kmr | This study |
| S-479S | S-497, Rfr, Kmr, Smr | This study |
| H-890 | HM21R, hgpB::Cmr, Rfr, Cmr, Smr | This study |
| SH-894 | S-497S, hgpB::Cmr, Rfr, Kmr, Cmr, Smr | This study |
| HM21TP | HM21R, Tn7, Rf r, Tpr | This study |
| HM21C1 | HM21R, Tn7::hgpB, Rf r, Tpr | This study |
| HM21C2 | HM21R, Tn7::hgpBR, Rf r, Tpr | This study |
| SHTP | SH-894, Rf r, Kmr, Cmr, Smr, Tpr | This study |
| SHC1 | SH-894, Tn7::hgpB, Rf r, Kmr, Cmr, Smr, Tpr | This study |
| SHC2 | SH-894, Tn7::hgpBR, Rf r, Kmr, Cmr, Smr, Tpr | This study |
| SHC3 | SH-894, Tn7::hgpB+intergenic regions,Rf r, Kmr, Cmr, Smr, Tpr | This study |
| SHC4 | SH-894, Tn7::hgpR, Rf r, Kmr, Cmr, Smr, Tpr | This study |
| E. coli STRAINS | ||
| S17-1 λ pir | Strain used for conjugation with A. veronii | de Lorenzo and Timmis, 1994 |
| BW20767 | Conjugation donor strain | Boccazzi et al., 2000 |
| DH5 αλ pir | Cloning strain capable is maintaining suicide vectors | Boccazzi et al., 2000 |
| Top10 | Cloning strain; Kmr | Invitrogen |
| PLASMIDS | ||
| pBC SK | Cmr | Stratagene |
| pKAS46 | pGP704, rpsL, Kmr | Skorupski and Taylor, 1996 |
| pCR2.1 | lac promoter, lacZα fragment, Apr, Kmr, pUC ori, fl origin | Invitrogen |
| pMM1 | pCR2.1, Cmr | This study |
| pMM2 | pCR2.1, 1.5 kb hgpB fragment | This study |
| pMM3 | pCR2.1 1.5 kb hgpB fragment, Cmr | This study |
| pMM4 | pKAS46, 1.5 kb hgpB fragment, Cmr | This study |
| pUC18R6KT-miniTn7T | Apr, R6K replicon, oriT, Tn7 | Choi et al., 2005 |
| pFTP1 | Cassette vector, Apr, Tpr, source of TPr cassette | Choi et al., 2005 |
| pTn7Tp | Apr, Tpr, R6K replicon, oriT, Tn7 | This study |
| pTnHA | Apr, Tpr, R6K replicon, oriT, Tn7::hgpB | This study |
| pTn7C2 | Apr, Tpr, R6K replicon, oriT, Tn7::hgpBR | This study |
| pEVS104 | Helper Tra, Trb, Kmr Apr, R6K replicon; | Stabb and Ruby, 2002 |
| pTNS2 | Encodes the TnsABC+D specific transposition pathway | Choi et al., 2005 |
| pRL27 | Kmr, modified Tn5 plasposon | Larsen et al., 2002 |
| pBBL3 | Kmr, carries transposon insertion and flanking DNA from S-497 | This study |