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. 2015 Mar 9;158(2):115–125. doi: 10.1093/jb/mvv028

Fig. 7.

Fig. 7

Increased membrane-association of the mutant RPE65s at low temperature. Total cell lysates and membrane fractions of 293 T-LC cells expressing WT or mutant RPE65 were analyzed by immunoblot analysis using antibodies against RPE65, β-actin or E-cadherin (CDH1). Histograms show relative contents of RPE65 proteins in the indicated fractions of cells maintained at 37 versus 30 °C. Contents of RPE65 in the cell lysates and membrane fractions were normalized by β-actin or CDH1 levels, respectively. Error bars show SD (n = 3).