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AVLE upregulated phosphorylated eNOS (A) and Akt (B) protein expression in a concentration-dependent manner (0, 0.3, 1 and 10 µg/mL) in HUVECs. AVLE (10 µg/mL) steadily upregulated phosphorylated eNOS (C) and Akt (D) protein expression in HUVEC in interval 30 min time points. Results are means ± SEM (n = 3). * p < 0.05 compared to control.
