Table 1.

Kinetic parameters of porcine pancreatic lipase obtained by fitting Equations (2) and (3) to the experimental data. Both equations were fitted simultaneously to the four substrate saturation curves obtained with the different pinhão coat extract concentrations shown in Figure 2. A non-linear least-squares procedure was used. The error terms correspond to standard deviations of the optimized parameters. The model selection criterion was 4.448, the correlation coefficient 0.995 and the sum of squared deviations 0.0541.

Parameters	Optimized Values
	Equation (2)	Equation (3)
KM (mM)	0.0583 ± 0.0077	0.0581 ± 0.0068
Vmax (μmol·min−1)	2.094 ± 0.126	2.079 ± 0.112
KiS (mM)	1.401 ± 0.337	1.418 ± 0.311
Ki1 (μg·mL−1)	97,620 ± 15,080,211	—
${\mathrm{K}}_{\mathrm{i}1}^{\prime }$ (μg·mL−1)	1.090 ± 168.991	—
Ki2 (μg·mL−1)	1604.5 ± 1887.5	—
${\mathrm{K}}_{\mathrm{i}2}^{\prime }$ (μg·mL−1)	82.19 ± 121.9	—
${\overline{\mathrm{K}}}_{\mathrm{i}1}$ (μg·mL−1)	—	332.7 ± 146.1
${\overline{\mathrm{K}}}_{\mathrm{i}2}$ (μg·mL−1)	—	321.2 ± 93.0
Sum of squared deviations	0.0614	0.0541
Model selection criterion	4.210	4.448