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. 2015 Jul 28;6:382. doi: 10.3389/fimmu.2015.00382

Figure 6.

Figure 6

Histone-specific T cell lines cross react to mAbs with CDR3 mimotopes. Th cell lines were cultured with irradiated APC and peptides in 10-day cycles, see Materials and Methods. (A) Micrographs Th cell lines (TCL): Left second cycle Th cells from DTG mice responding to HisH2A-ep1 peptide, Th cell blasts and proliferation clusters are seen. Middle left: third cycle Th cells from DTG mice responding to Id-peptide, only few Th cells are seen (arrows) admixed with APC. Middle, right: third cycle His2A-ep1 TCL Th cell line from L2-TG mice, sparse Th cells (arrows) are indicated. Right: third cycle His2A-ep1 TCL from BALB/c. (B) Analysis of peptide specific TCL, cross reaction to mAbs: TCL lines from DTG, BALB/c and L2-TG were tested for responses toward mAbs with IgVH CDR3 sequence similarity with histone peptides. 5.3 mAb: IgVH CDR3 similar to His4Ep1. 21.5: IgVH CDR3 similar to His2A-ep1. Polyclonal mouse IgG was control. Shown is proliferative response toward appropriate peptide or mAbs (n = 4, Student’s t-test).