Figure 3.

(A) Co-distribution of E7 with the cell membrane marker CellMask Orange (red) in unpermeabilised SiHa cells. Live cells were dual-stained with anti-E7 (Abcam) and CellMask Orange; (B) Time and temperature-dependent internalisation of cell surface E7, chased by anti-E7 antibody (Abcam). SiHa cells were incubated with the anti-E7 antibody for 1 h at 4 °C and incubated at 37 °C for 30 min to allow E7 internalisation. Cells were fixed, permeabilised and stained with AlexaFluor568 anti-mouse antibodies. Fluorescent imaging was performed using either a Zeiss LSM700 or LSM510 inverted microscope. The scale bar = 10 µm.