Log10[BeeMLV] titers of genomic (g) and sub-genomic plus genomic (sg + g) BeeMLV RNA in pupae (Pu), adults (Ad) and varroa (V) samples collected in Autumn 2002 from four apiaries in Western (#208; blue), Northern (#28; green) Eastern (#802; orange) and Southern (#878; red) France. Values represent the mean and standard deviation of two independent assays for only genomic RNA, and two assays for genomic-plus-subgenomic RNA (located 5′ and 3′ respectively of the putative start of the sub-genomic RNA; Figure 1), run on the pooled cDNA from 10 colonies in each apiary, for each of the sample types. The faded area represents the limit of detection. Significant differences between the genomic and genomic-plus-subgenomic titers, as determined by t-tests, are marked by an asterisk (*).