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. 2015 Jul 17;7(7):3891–3909. doi: 10.3390/v7072803

Figure 3.

Figure 3

Biological characteristics of CC024 vaccine strain. (a) Concentrated virus was layered onto 20%–60% sucrose gradients and subjected to ultracentrifugation as described in Materials and Methods. Nineteen fractions were taken from the top to bottom and assayed. The odd fractions were analyzed by Western blot using a CA16 polyclonal antibody; (b) Viral RNAs of peaks at F9 and F14 were detected by RT-PCR; (c) Viral titers of peaks at F9 and F14 were detected in Vero cell lines; (d,e) Electron microscopy of CC024 virus from peaks at F9 and F14. Bar = 200 nm.