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Mx1 induction in porcine cells transfected with the 3′NCR RNA. SK6 cells (1 × 106) were transfected with 20 μg/mL 3′NCR transcripts. Cells were lysed at 0, 6, 24 or 30 h after transfection. (A) RT-PCR detection of IFN-β, Mx1 and GAPDH mRNAs in RNA extracted from SK6 lysates. Negative controls (water) were included in the RT-PCR assays; (B) Mx1 detection by immunoblot in transfected SK6 cells. Tubulin was used for normalization.
