Figure 4.

Innate responses in transfected PBMCs from different pigs and two independent experiments. (A) PBMCs were isolated from five 9 to 12-month-old pigs and transfected with 20 μg/mL S, IRES, 3′NCR, mock-transfected (negative control), or stimulated with ODN (10 μg/mL) or poly I:C (10 μg/mL) (n = 2). The levels of IFN-α, TNF-α, IL-12 and IL-10 were measured 24 h later by ELISA. Data correspond to individual animals for each group and analysis; (B) PBMCs were isolated from six three-month-old pigs and transfected with IRES, 3′NCR, or mock-transfected as above. Supernatants were tested for antiviral activity (expressed as in Figure 3) at 24 h pt. Individual pig numbers are indicated.