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. 2015 Jul 17;7(7):3954–3973. doi: 10.3390/v7072807

Figure 6.

Figure 6

Effect of BafA1 treatment on cytokine induction in ncRNA-transfected PBMCs. Porcine PBMCs were treated with bafilomycin A1 (250 nM) 15 to 30 min prior to transfection or stimulation. IFN-α, TNF-α and IL-12 levels were measured by ELISA in total PBMCs 24 h after transfection with the ncRNAs (20 μg/mL) or stimulation with ODN (10 μg/mL) or LPS (0.5 μg/mL). Mock-transfections with medium were used as controls. Data are average values plus standard deviations from duplicates of two transfection experiments. Negative samples with OD readings ≤ the mean of blank wells were arbitrarily assigned values of 50, 25, and 100 for IFN-α, TNF-α, and IL-12, respectively, according to the detection limit for each cytokine.