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. 2015 Jul 21;7(7):4119–4130. doi: 10.3390/v7072812

Figure 1.

Figure 1

Liposome floatation assay of wild type and mutant NS4A(1–48). Alexa-488-labeled NS4A(1–48) (A); Alexa-488-labeled NS4A(1–48, L6E;M10E) (B); or free Alexa Fluor 488 dye (C) were mixed with sonicated POPC liposomes and loaded with the 35% (w/v) sucrose layer of a sucrose step gradient schematically shown on the left; Alexa-488-labeled NS4A(1–48) without liposomes was loaded with the 35% (w/v) sucrose layer in lane (D). Note: The narrow green line at the top of tubes (B), (C) and (D) results from reflection of fluorescence light at the air buffer interface rather than the presence of dye. Fluorescence images of the four tubes recorded prior to ultracentrifugation are shown in the lower row.