Differential mapping of sequence reads to the mitochondrial chromosome. (A) Chemical structure of the cation in compound 1 (the anion PF6− is not shown). (B) Heat map showing chromosomal mapping of sequence reads (log10FPKM). From inside out (arrow), values are represented as the triplicate mean for each sample type: 0 h, control-exposed; 4, 12, 24, and 48 h, control- and compound-exposed. (C) Graph showing total number of reads mapped to the ChrM (FPKM) for control- and compound-exposed samples across the time series. Gray line = mean; *P < 0.05 (Welch two-sample t test).