Skip to main content
. 2015 Jul 10;112(29):E3800–E3805. doi: 10.1073/pnas.1500925112

Fig. 4.

Fig. 4.

Nonapoptotic response to compound 1 in A2780 cells. (A) Heat map showing the differential gene expression (as fold change, logFC) of apoptotic markers (FDR < 0.10) at four time points. (B) Heat map showing the differential protein levels of apoptotic markers in response to compound 1 at two doses at four time points. (C) Flow cytometry measurements for detecting apoptotic cell death: negative control (Left) and after exposure to 150 nM of compound 1 for 24 h (Right). (D) High-content microscopy images of cells exposed to 2.5 µM of compound 1 for 48 h using the NucView substrate. Green fluorescence identifies caspase 3 activation of NucView probe, with blue DAPI dye highlighting cell nuclei.