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Gefitinib paradoxically activates ERK1/2 in EGFR-mutated NSCLC cells after 6–24 h of incubation. Western blot analysis. Exponentially growing HCC827 cells were treated with various concentrations of gefitinib and collected after 1 h (A), 6 h (B), 12 h (C), and 24 h (D) of treatment.
