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. 2015 Jul 6;112(29):9112–9117. doi: 10.1073/pnas.1505411112

Fig. 3.

Fig. 3.

Adaptin-3 and the EMC sensitize cells to isometamidium. (A) Cumulative growth of AP-3 RNAi strains (AP-3β subunit: Tb927.11.10650). Error bars indicate SD calculated from two independent strains. (B) Cumulative growth of EMC RNAi strains (EMC2 subunit: Tb927.7.6260). Error bars as in A. (C) Dose–response curves for ISM in AP-3 RNAi strains. EC50 values are indicated. Error bars indicate SD calculated from two experiments carried out in triplicate. (D) Dose–response curves for ISM in EMC RNAi strains. Other details as in C. (E) Cells were stained with an acidocalcisome marker, α-VP1 (green), and counterstained with DAPI (blue). (Scale bars, 20 μm.) (C–E) RNAi strains were preinduced for 72 h in 2 ng/mL, 1 μg/mL, or 1 ng/mL tetracycline for the V-ATPase, AP-3, or EMC subunits, respectively.