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. 2015 Jun 29;112(29):E3816–E3825. doi: 10.1073/pnas.1510077112

Fig. 3.

Fig. 3.

Screening of cancer cell lines for the expressions of tRNA halves. (A, Left) A schematic representation of 5′-tRNA half quantification. (Right) The 5′-tRNAAspGUC half and 5′-tRNAHisGUG half in BT-474 and HeLa total RNA were quantified using a method with or without T4 PNK or T4 RNA ligase (T4 Rnl) treatment. (B, Left) A schematic representation of 3′-tRNA half quantification. (Right) The 3′-tRNAAspGUC half was quantified by using the method described for BT-474 and HeLa total RNA. (C) Expression levels of the 5′-tRNAAspGUC half, 5′-tRNAHisGUG, and 3′-tRNAAspGUC half in the indicated cancer cell lines were examined. Expression levels in BT-20 cells were set as 1, and fold-changes are indicated. Averages of three independent experiments with SD values are shown. (D) Total RNA from the indicated prostate cancer cell lines was subjected to Northern blot for detection of tRNA halves derived from tRNAAspGUC and tRNAHisGUG. Detected mature tRNAs and tRNA halves are indicated by arrows.