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. 2015 Jul 6;112(29):8953–8958. doi: 10.1073/pnas.1507606112

Fig. 2.

Fig. 2.

Refactoring of the Reb (rebeccamycin, GenBank accession no. KF551872) and Tam (tetarimycin, GenBank accession no. JX843821) gene clusters. (A) Both promoter regions in the Reb cluster were replaced with synthetic promoters. HPLC analysis of extracts from S. albus cultures containing either the refactored (i) or wild-type cluster (ii) indicates that these cultures produce comparable levels of rebeccamycin. (B) The Tam gene cluster encodes tetarimycin A (2) but is silent in S. albus (iii). The three bidirectional (P1–P3) and one unidirectional (P4) promoter region in the Tam gene cluster was exchanged with synthetic bidirectional promoter cassettes. HPLC analysis of extracts from S. albus cultures either transformed with the fully promoter refactored gene cluster (i) or the Tam gene clusters activated through expression of the tamI SARP gene (ii) indicates that these cultures produce comparable levels of tetarimycin A (2).