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. 2015 Jul 2;112(29):9100–9105. doi: 10.1073/pnas.1419225112

Fig. 1.

Fig. 1.

Genomic characteristics and organization of Aspergillus fumigatus tetramycovirus-1 (AfuTmV-1). (A) AfuTMV-1 was purified from Af293 mycelia, and dsRNA isolated using phenol/chloroform was fractionated on a 1% agarose gel, and the positions of dsRNAs 1–4 in lane 1 are shown. Lane M contains HyperLadder I kb DNA marker. (B) RNase III sensitivity of purified AfuTmV-1 and AfuTmV-1 dsRNA was investigated, respectively, and untreated samples of each are shown following agarose gel electrophoresis in lanes 1 and 3, with RNase III digestions of each shown in lanes 2 and 4. (C) Genome organization of AfuTMV-1 dsRNAs 1–4 showing putative ORFs and UTRs. The positions of motifs characteristic for RdRP and methyltransferase (SAM) are shown as gray boxes on dsRNA 1 and dsRNA 3, respectively. (D) Assignation of cDNA clones specific to dsRNAs 1–4 by northern hybridization. Purified viral dsRNAs were fractionated by electrophoresis in 1% agarose gel in 1× TAE, denatured, blotted onto nylon membrane, and probed with clones specific for each dsRNA as shown in C. Lane 1 shows the AfuTmV-1 dsRNA profile, and lanes 2–5, individual transfers hybridized with probes specific for dsRNAs 1–4, respectively.