Fig. S7.

Effect of lncRNA-CD244 knockdown on apoptosis of CD8⁺ T cells and GSTT1 expression and analysis of the association between GSTT1 expression and IFN-γ/TNF-α expression by CD8⁺ T cells. (A) qPCR analysis of GSTT1 mRNA expression in CD8⁺ T cells of patients with active TB that were transfected with siRNA-lncRNA and siRNA-Ctrl. (B and C) Pooled data showing the concentrations of IFN-γ and TNF-α determined by CBA analysis upon transduction of indicated LV containing shRNAs targeting GSTT1 (LV-GSTT1), control LV (LV-Ctrl), or medium. (D and E) Representative flow cytometric analysis (D) and pooled data of five TB patients (E) of CD8⁺ T cells transfected with siRNA-lncRNA-CD244 or siRNA-Ctrl at day 5 and then analyzed for apoptosis based on the intracellular expression of Annexin V and propidium iodide (n = 5). NS, no statistically significant difference. Error bars represent SEM from three independent experiments.