Fig. S8.

RIP analysis shows that lncRNA-CD244 interacts with EZH2 upon exogenous expression in HEK293T cells. lncRNA-CD244 vector and EZH2-EGFP vector were cotransfected into HEK293T cells. Extracts of HEK293T cells were prepared, and EZH2 protein was immunoprecipitated by either IgG control antibody or anti-EZH2–specific antibody and tested for association with lncRNA-CD244 by qRT-PCR. (A and B) Gel electrophoresis (A) and qPCR analysis (B) of lncRNA-CD244 retrieve in IP by IgG or anti-EZH2–specific antibody from lysates of HEK293T cell. The levels of qRT-PCR products were expressed as a percentage of input RNA. ***P < 0.001. Error bars represent SEM from three independent experiments.