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. 2015 Apr 24;1(3):e1500175. doi: 10.1126/sciadv.1500175

Fig. 1. N-α-PGP activates endothelial cell signaling through CXCR2.

Fig. 1

(A to C) HUVECs were serum-starved for 2 hours before stimulation with N-α-PGP (0.5 mg/ml) for indicated times, and activation of Rac1 (A) and phosphorylation of PAK (pPAK) and ERK (pERK) (B) and VE-cadherin (pVE-cad) (C) were determined by Western blot. Shown are representative Western blots together with quantification. Bar graphs show means ± SEM (n = 3). *P < 0.05 relative to time 0 by one-way analysis of variance (ANOVA) with Tukey post-test. (D) HUVECs were untreated or treated with N-α-PGP (0.5 mg/ml) (30 min) alone or after pretreatment with 200 nM SB225002, and Rac1 activity and phosphorylation of ERK, PAK, and VE-cadherin were determined by Western blot. Shown are representative Western blots together with quantification. Bar graphs show means ± SEM (n = 3). *P < 0.05 relative to time 0, #P < 0.05 relative to PGP by one-way ANOVA with Tukey post-test.