Table A1.

Primer Pairs Used for Site-Directed Mutagenesis in Generation of Adrenocortical Carcinoma–Associated TP53 Mutations

Variant	Codon	Forward Primer	Reverse Primer
P72R	72	ggctgctccccgcgtggcccctg	caggggccacgcggggagcagcc
F134Y	134	gccctcaacaagatgtattgccaactggccaag	cttggccagttggcaatacatcttgttgagggc
R158L	158	cacccgcgtcctcgccatggcca	tggccatggcgaggacgcgggtg
R158H	158	cacccgcgtccacgccatggcca	tggccatggcgtggacgcgggtg
Y163C	163	cgccatggccatctgcaagcagtcacagc	gctgtgactgcttgcagatggccatggcg
R175L	175	gaggttgtgaggcactgcccccaccat	atggtgggggcagtgcctcacaacctc
R175H	175	gaggttgtgaggcactgcccccaccat	atggtgggggcagtgcctcacaacctc
H193P	193	ggcccctcctcagcctcttatccgagtgg	ccactcggataagaggctgaggaggggcc
F212I	212	ttggatgacagaaacactattcgacatagtgtggtgg	ccaccacactatgtcgaatagtgtttctgtcatccaa
P219S	219	catagtgtggtggtgtcctatgagccgcctg	caggcggctcataggacaccaccacactatg
C229R	229	gaggttggctctgaccgtaccaccatccact	agtggatggtggtacggtcagagccaacctc
S241Y	241	tacaactacatgtgtaacagttactgcatgggcgg	ccgcccatgcagtaactgttacacatgtagttgta
R248W	248	gggcggcatgaactggaggcccatcc	ggatgggcctccagttcatgccgccc
R248L	248	ggcggcatgaacctgaggcccatcctc	gaggatgggcctcaggttcatgccgcc
I254T	254	ggcccatcctcaccaccatcacactggaaga	tcttccagtgtgatggtggtgaggatgggcc
R267Q	267	tggtaatctactgggacagaacagctttgaggtgc	gcacctcaaagctgttctgtcccagtagattacca
R273H	273	gaacagctttgaggtgcatgtttgtgcctgtcctg	caggacaggcacaaacatgcacctcaaagctgttc
C275X	275	ctttgaggtgcgtgtttgaggtgctgggagagaccggc	gccggtctctcccagcacctcaaacacgcacctcaaag
E285V	285	gaccggcgcacagtggaagagaatctcc	ggagattctcttccactgtgcgccggtc
Ins933	311	gcgagcactgcccaacaaacaccagctcc	ggagctggtgtttgttgggcagtgctcgc
R337H	337	cgtgggcgtgagcacttcgagatgttccgag	ctcggaacatctcgaagtgctcacgcccacg
R337H	337	ccgtgggcgtgagcacttcgagatgttcc	ggaacatctcgaagtgctcacgcccacgg
C275X-GFP	G275	gctttgaggtgcgtgttcgggatccaccg	cggtggatcccgaacacgcacctcaaagc
Ins933a	G311	gatccgtgggcgcgggatccaccg	cggtggatcccgcgcccacggatc
Ins933a-GFP	G-933-b	gatccgtgggcgtgggatccagcgcttcgagatg	catctcgaagcgctggatcccacgcccacggatc