Figure 5.

Increasing concentrations of isoeugenol induce calcein leakage from large unilamellar vesicles of E. coli polar lipid extract. Vesicles with encapsulated calcein were suspended in MES-buffer (pH 6.0) and exposed to increasing concentrations of isoeugenol, either expressed as concentration (mg/mL, lower axis) or as a function of isoeugenol to lipid concentration ([IE]/[L], upper axis). Calcein intensity signal was measured before and after 1 h treatment at 37°C, and normalized to the signal obtained at full permeabilization for individual [IE]/[L] ratios. A lipid concentration of ~12 nmol was used for all [IE]/[L] ratios.