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. 2015 Jul 28;6:754. doi: 10.3389/fmicb.2015.00754

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Copyright © 2015 Hyldgaard, Mygind, Piotrowska, Foss and Meyer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-series of DOPG:DOPC (20:80) giant unilamellar vesicles (GUVs) before and during treatment with isoeugenol. Confocal laser scanning microscope images were sequentially acquired of the same field-of-view of GUVs stained with the membrane-bound dye Alexa 488 dextran (green) and the intravesicular water-soluble dye Alexa Fluor 633 hydrazide (red). Untreated GUVs (A) remained stable and intact until isoeugenol came in contact with GUVs (B). (C–F) At later time-points the interaction between isoeugenol and GUVs results in four distinct structural changes of vesicles: Fluctuation of vesicles (not indicated in images), release of intravesicular GUVs (white arrows), affected GUV shape with diverse outcomes (yellow arrows), and tubular or bead protrusions from GUVs (red arrows). Bars correspond to 20 μm.