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. 2015 Jul 28;5:12521. doi: 10.1038/srep12521

Figure 6. MBNL1 decreases non-ATG-initiated translation of an expHTT RNA fragment.

Figure 6

SK-N-MC cells were co-transfected with N63(CAG)66HTT-RAN and GFP-MBNL1 plasmids, and 72 hours post-transfection levels of polyAla-containing protein from RAN translation were assessed by western blot. GFP plasmid was used as a control. Overexpression of MBNL1 decreased the level of polyAla RAN protein, while the MBNL1 Δ12–46 and GFP-MBNL1 Δ251–388 deletions increased the expression of polyAla-containing RAN product. One-way ANOVA, n = 3 biological replicates. *P < 0.05, versus GFP group.