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. 2015 Jul 28;6:761. doi: 10.3389/fmicb.2015.00761

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Copyright © 2015 Huedo, Yero, Martinez-Servat, Ruyra, Roher, Daura and Gibert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) T-seeded colony-based DSF bioassay of strains E77 and M30. Arrow indicates DSF production of M30 (rpf-2) strain upon detection of DSF molecules produced by E77 (rpf-1). (B) DSF quantification of supernatants from axenic cultures of E77 and M30, a mixed culture E77:M30 (1:1), an axenic culture of M30 supplemented with synthetic DSF at 0.05 μM final concentration and a corresponding control (equal volume of LB broth containing 0.05 μM DSF), using the microtitter DSF bioassay; ^*p < 0.05 by One-Way ANOVA and post-test. (C) Colony-based DSF bioassay of E77, M30 and their respective ΔrpfF mutants seeded at different distances on the same agar plate.