Figure 4. dpp-Gal4 driven expression of Nact and Src42ACA also upregulates MMP1 and induces apoptosis.
UAS transgenes as indicated were driven with dppGal4 along with UAS-GFP at 18°C. Controls express an extra copy of UAS-GFP. Wing discs were stained with anti-MMP1 (A–L) or anti-cleaved caspase 3 (cl-casp3, M–X). The combination of Nact and Src42ACA induces both MMP1 (A, G) and cl-casp3 (M, S), and Src42ACA alone does the same to a lesser extent (B, H, N, T). Green arrows: GFP positive cells that do not express MMP1 (G, H) or cl-casp3 (S, T). Red arrows: cl-casp3-positive cells that do not express GFP, indicating a potentially non-cell-autonomous effect. This effect can be largely rescued with BskDN (E, K, Q, W). Similarly, the combination of Src64B and Nact also induces both MMP1 (F, L) and cl-casp3 (R, X). Scale bar: 100 μM.