Figure 7. Notch targets are differentially affected by N/Src synergy.
(A) qPCR assay for expression levels of E(spl) complex members in vgGal4 wing discs expressing UAS constructs as indicated. (B–E) Immunostaining with anti-cut (red) in vgGal4 wing discs. Nact alone (D) induces cut expression, which is suppressed in Nact+Src42ACA discs (B). Note that both ectopic and endogenous cut appear to be suppressed. (F) NRE-GFP expression in wing discs expressing Nact+Src42ACA under vgGal4. Scale bar: 100 μm.
Figure 7—figure supplement 1. E(spl)mγ reporter staining in N/Src wing discs.
VgGal4 wing discs expressing UAS-Nact and/or UAS-Src42ACA in an E(spl)mγ-LacZ/+ background were stained for anti-β-gal. E(spl)mγ-LacZ consists of the 234-bp mγ enhancer region fused to a LacZ reporter (Nellesen et al., 1999). Note that E(spl)mγ induced by Nact (C) is not suppressed by the addition of Src42ACA (A). Src42ACA alone (B) seems to suppress the endogenous E(spl)mγ staining (D) in the proneural cells.