(A) Bone marrow-derived macrophages from wild-type mice were transfected with siRNA targeting Abca1, Abcg1 or control (Ctrl) for 48 hr, pretreated with GW3965 (1 µM) overnight, and then stimulated with LPS (10 ng/ml) for 4 hr. (B, C) Bone marrow-derived macrophages from myeloid-specific Abca1−/− and control wild-type mice were pretreated with GW3965 (1 µM) overnight, followed by stimulation with LPS (10 ng/ml) for 4 hr. Gene expression was analyzed by real-time PCR (B) and Agilent microarrays (C). Selected genes from the array studies that are annotated with the ‘Immune system process’ GO term are presented as a heatmap (≥twofold changes shown). N = 4 per group. *p < 0.05, **p < 0.01, NS, not significant. Error bars represent means ± SEM.
DOI:
http://dx.doi.org/10.7554/eLife.08009.008