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. Author manuscript; available in PMC: 2015 Jul 28.
Published in final edited form as: Hypertension. 2008 May 12;52(1):143–149. doi: 10.1161/HYPERTENSIONAHA.107.101667

Figure 2.

Figure 2

Effect of Nox4/p22-phox cotransfection on cellular mRNA levels. Adventitial myofibroblasts were transfected with either an empty-vector control plasmid or cotransfected with plasmids expressing human Nox4 and p22-phox. The cells were stimulated with either Ang II (100 nmol/L for 3 hours) or vehicle and mRNA levels assessed by conventional PCR. A, Agarose gels of PCR products using human Nox4 and p22 primers and rat 18sRNA primers. B, Quantification of band intensity using ImageJ software. Nox4 and p22-phox mRNA levels are shown by the shaded and filled bars, respectively. Data are expressed as fold change±SEM from their corresponding controls (empty vector plus vehicle; n=8 to 9). *Statistical significance where P<0.05.

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