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. Author manuscript; available in PMC: 2015 Jul 28.
Published in final edited form as: Hypertension. 2008 May 12;52(1):143–149. doi: 10.1161/HYPERTENSIONAHA.107.101667

Figure 3.

Figure 3

Effects of Nox4/p22-phox expression on endogenous levels of Nox4 mRNA. Adventitial myofibroblasts were transfected with either plasmids expressing human Nox4 and p22- phox or an empty vector control. Then they were stimulated with either Ang II (100 nmol/L) or vehicle, and mRNA levels for endogenous Nox4 and 18sRNA were determined using real-time PCR with rat-specific primers and probes. Data are expressed as the ratio of Nox4 mRNA vs 18sRNA and were plotted as a percentage of empty vector plus vehicle control on the day of assay±SEM. * P<0.05; n=6.

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