Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 28;6:760. doi: 10.3389/fmicb.2015.00760

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Thompson and Visick.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Protein production under SypG- and RscS-induced biofilm conditions. (A) HA-tagged derivatives of SypA (lanes 1–4) and SypA_VP (lanes 5–8) produced under rscS- (pARM7) or sypG- (pCLD56) overexpressing conditions and detected by Western blotting with anti-HA antibody. Lanes: 1 and 5, pARM7/ΔsypA (KV6578 and KV7315), 2 and 6, pARM7/ΔsypA ΔsypE (KV6580 and KV7316), 3 and 7, pCLD56/ΔsypA (KV6578 and KV7315) and 4 and 8, pCLD56/ΔsypA ΔsypE (KV6580 and KV7316). Lanes were loaded with 5 μl of whole cell lysate from cultures standardized to an OD₆₀₀ = 3. (B) HA-tagged derivatives of mutant SypA proteins produced under rscS- (pARM7) overexpression conditions: G25A (KV7560); R27A (KV7613); E71A (KV7566); K72A (KV7616); D34A (KV7562); R74A (KV7568); K90A (KV7620); P99A (KV7572); E2A (KV7558); G83A (KV7570); R93A (KV7010); D47A (KV7606); D55A (KV7607); Y64A (KV7564); Y66A (KV7615); K67A (KV6995); R68A (KV7005); D73A (KV7612); Q84A (KV7000). All lanes were loaded with 5 μl of whole cell lysate from cultures standardized to an OD₆₀₀ = 3.