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. 2015 Jul 28;6:760. doi: 10.3389/fmicb.2015.00760

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Copyright © 2015 Thompson and Visick.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Conserved residues in SypA function in biofilm formation. Development of colony morphology over time of rscS (pARM7)-overexpressing derivatives of ΔsypA strains that contain (A) the empty cassette (negative control; KV5079), or expressing (B) sypA (KV6578), (C) sypA-R27A (representative Class I mutant; KV7613), (D) sypA-D34A (representative Class II mutant; KV7562), (E) sypA-R93A (representative Class III mutant; KV7010), or (F) sypA-K67A (representative Class IV mutant; KV6995). Cultures were spotted onto LBS plates containing tet, and the morphologies of the resulting colonies were assessed at the indicated times. Representative images are shown.