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. 2015 Jul 28;6:760. doi: 10.3389/fmicb.2015.00760

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Copyright © 2015 Thompson and Visick.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SypA-R27A is resistant to control by SypE. Development of colony morphology over time of sypG (pCLD56)-overexpressing derivatives of ΔsypA (sypE+) strains that contain (A) native sypA (susceptible to SypE; KV6587), (B) sypA-S56A (expressing a mutant SypA that cannot be phosphorylated by SypE; KV6579), or the Class I mutants as follows: (C) sypA- G25A (KV7560), (D) sypA-E71A (KV7566), (E) sypA-R27A (KV7613), and (F) sypA-K72A (KV7616). Cultures were spotted onto LBS plates containing tet, and the morphologies of the resulting colonies were assessed at the indicated times. Representative images are shown.