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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2015 May 19;85:288–294. doi: 10.1016/j.freeradbiomed.2015.05.011

Figure 1. HASMCs reorient after mechanical stimulation.

Figure 1

HASMCs between passages 6 and 12 were seeded in BioFlex culture plates coated with type I collagen. Cells were subjected to repeated biaxial stretching cycles of 10% with a frequency of 1 Hz for 24h and them fixed and stained. Cell alignment was estimated using a fluorescence microscope as is described in Material and Methods. Representative image and bar graphs (mean ± SE) of at least 5–7 independent experiments performed in duplicate are shown. (*) indicates P<0.05.