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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2015 May 19;85:288–294. doi: 10.1016/j.freeradbiomed.2015.05.011

Figure 2. H2O2 mediates HASMC reorientation after mechanical stimulation.

Figure 2

Figure 2

Figure 2

HASMCs were seeded in BioFlex culture plates coated with type I collagen. A) Cells were subjected to repeated biaxial stretching cycles of 10% with a frequency of 1 Hz for 24h in phenol-red free media supplemented with 50 μM Amplex and 0.1 U/mL HRP. At the indicated times, H2O2 was quantified using a fluorometer as indicated in Materials and Methods. B and C) Cells were serum deprived and pretreated for four hours with SOD (B) or catalase (C) prior to 24 hours of cyclic stretch. After fixation and staining, cell alignment was estimated as indicated in Materials and Methods. Representative image and bar graphs (mean ± SE) of at least 5–7 independent experiments performed in duplicate are shown. * and ¥ Indicates P<0.05 for stretch and catalase, respectively.