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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2015 May 19;85:288–294. doi: 10.1016/j.freeradbiomed.2015.05.011

Figure 3. Nox4 is required for HASMC reorientation after mechanical stimulation.

Figure 3

Figure 3

HASMCs were transfected with siRNA against Nox4 or control non-targeting siRNA 3 days before mechanical stimulation. After 24 hours of stretch, cells were either collected for western blots to verify the downregulation of Nox4 (A), or fixed and stained to estimate cellular alignment (B) as indicated in Materials and Methods. Representative image and bar graphs (mean ± SE) of at least 5–7 independent experiments performed in duplicate are shown. * and ¥ Indicate P<0.05 for siRNA against Nox4 and stretch, respectively.