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. Author manuscript; available in PMC: 2016 Aug 1.

Published in final edited form as: Free Radic Biol Med. 2015 May 19;85:288–294. doi: 10.1016/j.freeradbiomed.2015.05.011

HASMCs were transfected with a plasmid containing a constitutive active form of LIMK or empty vector 48 hours before mechanical stimulation (A) or siRNA against Slingshot-1L phosphatase or non-targeting siRNA (B) 3 days before initiating mechanical stimulation. After 24 hours of stretch, cells were fixed and stained to estimate cellular alignment as indicated in Materials and Methods. Representative image and bar graphs (mean ± SE) of 4 independent experiments performed in duplicate are shown. (*) Indicates P<0.05 for the effect of stretch and ¥ indicates P<0.05 for the effect of LIMK and siSSH in A and B, respectively.

HASMCs were transfected with a plasmid containing a constitutive active form of LIMK or empty vector 48 hours before mechanical stimulation (A) or siRNA against Slingshot-1L phosphatase or non-targeting siRNA (B) 3 days before initiating mechanical stimulation. After 24 hours of stretch, cells were fixed and stained to estimate cellular alignment as indicated in Materials and Methods. Representative image and bar graphs (mean ± SE) of 4 independent experiments performed in duplicate are shown. (*) Indicates P<0.05 for the effect of stretch and ¥ indicates P<0.05 for the effect of LIMK and siSSH in A and B, respectively.