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. 2015 Jul 28;5:167. doi: 10.3389/fonc.2015.00167

Figure 2.

Figure 2

PEITC causes ROS accumulation only in ovarian cancer cell lines, not in normal OSE (ovarian epithelial cells). Normal OSE and cancer cell lines (PA-1 and SKOV-3) were incubated with either vehicle or 5 μM of PEITC for 12 or 24 h and ROS accumulation was detected by flow cytometry using 25 μM DCFH-DA (A). Cells were collected after PEITC treatment for 24 h, and GSH level was measured using glutathione assay kit (B). Values are means ± SEM (**P < 0.01 vs. vehicle control).