Skip to main content
. 2015 Jul 28;5:167. doi: 10.3389/fonc.2015.00167

Figure 5.

Figure 5

PEITC induces unfolded protein response with attenuation of UPR by antioxidant, NAC, in PA-1 and SKOV-3 ovarian cancer cells. Cells were harvested at indicated hours after 5 μM PEITC treatment. Western blotting was performed to detect three arms of UPR (P-PERK, IRE1α, and ATF-6), molecular chaperone GRP78 and GADD153 in (A). With or without 1 h pretreated 10 mM NAC. C, control (DMSO, the vehicle) group; N, NAC-treated group; P, PEITC-treated group for 24 h; N + P, NAC- and PEITC-treated group. NAC was treated for 1 h before 5 μM PEITC treatment (B). P-PERK, IRE1α, ATF-6, GADD153, and GRP78 bands were determined by densitometry of bands using ImageJ software. We analyzed statistical difference of triplicate experiments using ANOVA with Bonferroni’s post hoc test and performed using SPSS 20.0 software (C,D). Values are means ± SEM (*P < 0.05, **P < 0.01 vs. the vehicle control).