Fig. 10.

Suppression effects of calipteryxin and (3’S,4’S)-3’,4’-disenecioyloxy-3’,4’-dihydroseselin on the mRNA expression of the pro-inflammatory cytokines TNF-α (a) and IL-1β (b). Total RNA was isolated, and the expression of TNF-α and IL-1β was determined through qRT-PCR, as described in the “Methods.” Con (vehicle), LPS; (LPS + vehicle)-treated cells alone and TPCK (30 μM) served as a positive control. (***) p <0.001 indicates significant differences from the LPS-treated group. (^###) p <0.001 indicates a significant difference from the unstimulated control group