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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Immunol. 2015 Feb 25;194(7):3079–3087. doi: 10.4049/jimmunol.1402190

FIGURE 5.

FIGURE 5

miR-27a regulates IL-10 secretion via ERK activation. (A) Healthy monocytes were stimulated with 25 mM alcohol and/or HCV concentrate. Phosphorylated ERK levels were determined by flow cytometry. Representative dot plot for each treatment condition is shown. (B and C) Healthy monocytes were transfected with anti–miR control or miR-27a inhibitor (B) or miR control or miR-27a mimic (C), stimulated with 25 mM alcohol and/or HCV concentrate for 7 d as indicated. Phosphorylated ERK levels were determined by flow cytometry, and the data are represented as means ± SEM (n = 3). *p < 0.05. #p < 0.05 as compared with anti–miR control or miR control. (D–I) Monocytes were pretreated with various doses of U0126 and then stimulated with alcohol and HCV as indicated. (D–G) Cell surface expression of CD14, CD68, CD206, and DC-SIGN were determined on the monocytes by flow cytometry. The bar graphs represent the MFI of the indicated proteins. (H and I) Cell culture supernatants were tested for IL-10 and TGF-β secretion by ELISA. The data are represented as means ± SEM (n = 4 experiments). *p < 0.05. #p < 0.05 compared with the respective 0 μM U0126 control. EtOH, ethanol.