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. Author manuscript; available in PMC: 2015 Jul 28.
Published in final edited form as: J Neurochem. 2011 Jan 19;116(4):659–668. doi: 10.1111/j.1471-4159.2010.07158.x

Fig. 3. Lithium reduces LC3-II level in SH-SY5Y CLN3 knockdown and CbCln3Δex7/8/Δex7/8 cerebellar cells.

Fig. 3

(A) SH-SY5Y CLN3 AS cells were left untreated (Mock) or treated for 7 days with 10 mM NaCl (control) or 10 mM LiCl. Cell extracts were then prepared and analyzed by Western blotting with anti-LC3 antibody. (B) CbCln3Δex7/8/Δex7/8 cerebellar cells were left untreated (Mock) or treated for 7 days with 0.2 µM rapamycin, 10 mM NaCl (control), 10 µM SB216763 (GSK inhibitor) or 10 mM LiCl and then analyzed as in (A). (C and D) CbCln3Δex7/8/Δex7/8 cerebellar cells were treated with 10 mM LiCl (C) or 10 mM NaCl (D) for the indicated times and analyzed as in (A). The LC3-II signals on the blot were quantified as described in Fig. 1D.