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. Author manuscript; available in PMC: 2015 Jul 28.
Published in final edited form as: J Neurochem. 2011 Jan 19;116(4):659–668. doi: 10.1111/j.1471-4159.2010.07158.x

Fig. 6. Knockdown of IMPase1 increases autophagic maturation in CbCln3Δex7/8/Δex7/8 cerebellar cells.

Fig. 6

(A–C) Effect of IMPase knockdown on autophagic maturation in CbCln3Δex7/8/Δex7/8 cerebellar cells. CbCln3+/+ and CbCln3Δex7/8/Δex7/8 cells were transiently co-transfected with mCherry-GFP-LC3 and either control shRNA (pSUPER) or IMPase1 shRNA (shIMPase1) for 48 h. Cells were then examined under a confocal microscope (A) and the numbers of cells showing only red fluorescence in A were counted (B). Bars indicate means ± S.D. (n > 20 cells) with significant difference from control (pSUPER) (p < 0.05). Expression level of IMPase1 was examined by quantitative RT-PCR (left) and Western blotting using IMPase1 antibody (right) (C).