Table 1.

Enzymes, substrates and reaction conditions for each proteinase tested

Enzyme	[Enzyme]	[Substrate] 250 μM	Activity buffer
Thrombin	74 nM	Boc-Val-Arg -AMC	50 mM Tris–HCl pH 8, 100 mM NaCl
Trypsin	54 nM	Boc-Gln-Ala-Arg-AMC	50 mM Tris–HCl pH 8, 1 mM CaCl2, 0.15 M NaCl
Cathepsin G	0.33 nM	N-Succinyl-Ala-Ala-Phe-AMC.	50 mM HEPES/NaOH pH 7.5
Cathepsin L	0.83 nM	Z-Phe-Arg-AMC	Sodium acetate 100 mM pH 5.5, 1 mM EDTA, 4 mM DTT, 0.001% BSA
Plasmine	333 nM	Nt-Boc-Val-Leu-Lys-AMC	50 mM Tris–HCl pH 8, 50 mM NaCl
Elastase	1.2 nM	N-Methoxysuccinyl-Ala-Ala-Pro-Val-7 amido AMC	25 mM Tris–HCl pH 8, 100 mM NaCl, 1 mM CaCl2
Chymotrypsin	0.012 nM	N-succinyl-Lelu-Leu-Val-Tyr-7 amido AMC	100 mM HEPES/ NaOH pH 7.5
Factor Xa	0.054 nM	Boc-Ile-Glu-Gly-Arg-7 amido AMC	50 mM Tris–HCl pH 8.3, 5 mM CaCl2, 0.2 mM NaCl
Factor XIa	14,000 nM	Boc-Phe-Ser-Arg-7 amido AMC	50 mM Tris–HCl pH 8, 100 mM NaCl, 1 mM CaCl2
Factor XIIa	0.0017 nM	Boc-Val-Arg-AMC	4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3